Hotspot

rps16-trnQ-UUG

LSC · 5749-6742 · 994 bp

Back to Driessenia

Pi 0.0218

MarkerSeek score 62.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 994
variable_sites: 32
pi: 0.0218
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1045-1059 bp	1.0	77.1
rps16-trnQ-UUG_p2	CGTTGCTTTCTACCACATCG	TTCGGAGGTTCGAATCCTTC	50.0	50.0	57.2	57.3	1049-1063 bp	1.0	72.4
rps16-trnQ-UUG_p3	CACGTTGCTTTCTACCACATC	GAGGTTCGAATCCTTCCGTC	47.6	55.0	57.5	58.1	1047-1061 bp	1.0	71.8
rps16-trnQ-UUG_p4	CGTTGCTTTCTACCACATCG	GGGTTTTGGTCCCGCTATTC	50.0	55.0	57.2	58.9	1066-1080 bp	1.0	70.4
rps16-trnQ-UUG_p5	CACGTTGCTTTCTACCACAT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	56.4	58.1	1047-1061 bp	1.0	70.3