Hotspot

ndhD

SSC · 110410-111912 · 1503 bp

Back to Drepanostachyum

Pi 0.00177

MarkerSeek score 59.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 4
pi: 0.00177
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CCACTATGGAATATAGAGCGGAC	CGGGCTTTTCTGGTCAAAAC	47.8	50.0	58.0	57.9	1583-1584 bp	1.0	55.2
ndhD_p2	CCACTATGGAATATAGAGCGGAC	GGGCTTTTCTGGTCAAAACG	47.8	50.0	58.0	57.9	1582-1583 bp	1.0	55.2
ndhD_p3	TCCACTATGGAATATAGAGCGGA	CGGGCTTTTCTGGTCAAAAC	43.5	50.0	58.1	57.9	1584-1585 bp	1.0	54.6
ndhD_p4	TCCACTATGGAATATAGAGCGGA	GGGCTTTTCTGGTCAAAACG	43.5	50.0	58.1	57.9	1583-1584 bp	1.0	54.6
ndhD_p5	CCACTATGGAATATAGAGCGGAC	ACGGGCTTTTCTGGTCAAAA	47.8	45.0	58.0	58.2	1584-1585 bp	1.0	54.4