Hotspot

psaJ-rpl33

LSC · 67266-67715 · 450 bp

Back to Drepanostachyum

Pi 0.0104

MarkerSeek score 59.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 7
pi: 0.0104
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTGGGGCTTTAGCAGGTTT	GAAATCCCCGTCGATTCCTC	45.0	55.0	57.9	58.1	612-618 bp	1.0	79.7
psaJ-rpl33_p2	TTTGGGGCTTTAGCAGGTTT	GGCACCTTTTCGAACACAAC	45.0	50.0	57.9	58.2	589-595 bp	1.0	79.5
psaJ-rpl33_p3	TTTGGGGCTTTAGCAGGTTT	CGATTCCTCATTGGCACCTT	45.0	50.0	57.9	58.2	601-607 bp	1.0	78.9
psaJ-rpl33_p4	TTTGGGGCTTTAGCAGGTTT	CTAACACCTTTCCCCTTGGC	45.0	55.0	57.9	58.5	543-549 bp	1.0	77.4
psaJ-rpl33_p5	TTTGGGGCTTTAGCAGGTTT	CCTCATTGGCACCTTTTCGA	45.0	50.0	57.9	58.5	596-602 bp	1.0	77.1