Hotspot

ycf4-cemA

LSC · 60692-61091 · 400 bp

Back to Drepanostachyum

Pi 0.0117

MarkerSeek score 62.00

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 400
variable_sites: 7
pi: 0.0117
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.9	57.8	504 bp	1.0	78.5
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	AGAGAAAGAGACTCCCCAGG	50.0	55.0	58.1	57.8	506 bp	1.0	78.2
ycf4-cemA_p3	ACAAAAAGCTGCCGAATTGG	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.8	57.8	533 bp	1.0	77.9
ycf4-cemA_p4	TTTCTTGCGCGTACCAATTG	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.7	57.8	509 bp	1.0	76.5
ycf4-cemA_p5	CCTATTTCTTGCGCGTACCA	AGAGAAAGAGACTCCCCAGG	50.0	55.0	58.4	57.8	513 bp	1.0	76.3