Hotspot

rbcL-psaI

LSC · 58373-59659 · 1287 bp

Back to Drepanostachyum

Pi 0.00259

MarkerSeek score 60.50

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1287
variable_sites: 5
pi: 0.00259
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AAATTTGAGTTCGAGCCGGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.1	58.0	1395-1396 bp	1.0	79.3
rbcL-psaI_p2	GCCGCAGCTTGTGAAATATG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	1425-1426 bp	1.0	78.8
rbcL-psaI_p3	AAATTTGAGTTCGAGCCGGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.1	57.9	1401-1402 bp	1.0	78.7
rbcL-psaI_p4	GCCGCAGCTTGTGAAATATG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	1431-1432 bp	1.0	78.2
rbcL-psaI_p5	CAAATTTGAGTTCGAGCCGG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1396-1397 bp	1.0	78.1