Hotspot

trnS-GUA-psbD

LSC · 8468-9447 · 980 bp

Back to Drepanostachyum

Pi 0.00408

MarkerSeek score 61.40

Primer available yes

Label trnS-GUA-psbD

Feature Metadata

label_name: trnS-GUA-psbD
feature_type: igs
region: LSC
length_bp: 980
variable_sites: 6
pi: 0.00408
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GUA-psbD_p1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1125-1129 bp	1.0	78.8
trnS-GUA-psbD_p2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1112-1116 bp	1.0	78.2
trnS-GUA-psbD_p3	GCTTTAGTCCACTCAGCCAT	GCCGGACCATCCTACAAAAA	50.0	50.0	57.6	58.2	1111-1115 bp	1.0	75.8
trnS-GUA-psbD_p4	ATTAGCAATCCGCCGCTTTA	GAAACGGTCCCTTCGTAACC	45.0	55.0	58.0	58.6	1104-1108 bp	1.0	75.6
trnS-GUA-psbD_p5	GCTTTAGTCCACTCAGCCAT	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.6	58.2	1098-1102 bp	1.0	75.2