Hotspot

ycf1

SSC · 126194-131632 · 5439 bp

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Pi 0.000613

MarkerSeek score 40.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5439
variable_sites: 5
pi: 0.000613
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCAATTGGTTTGATTCCTT	TCAAGGGAGCTATCCTTTTT	35.0	40.0	52.9	53.9	246 bp	1.0	40.7
ycf1_p2	TCCAATTGGTTTGATTCCTT	ATCAAGGGAGCTATCCTTTT	35.0	40.0	52.9	53.6	247 bp	1.0	40.6
ycf1_p3	ATCCAATTGGTTTGATTCCT	TCAAGGGAGCTATCCTTTTT	35.0	40.0	52.6	53.9	247 bp	1.0	40.6
ycf1_p4	AATCCAATTGGTTTGATTCCT	TCAAGGGAGCTATCCTTTTT	33.3	40.0	53.6	53.9	248 bp	1.0	40.6
ycf1_p5	ATCCAATTGGTTTGATTCCTT	TCAAGGGAGCTATCCTTTTT	33.3	40.0	53.6	53.9	247 bp	1.0	40.6