Hotspot

rps4

LSC · 45138-45743 · 606 bp

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Pi 0.0011

MarkerSeek score 47.00

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: LSC
length_bp: 606
variable_sites: 1
pi: 0.0011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	AATGAGATAGGGCACGAGGT	TGAGTTTTGCTTTGCTGATTCT	50.0	36.4	58.2	57.1	784 bp	1.0	55.1
rps4_p2	TGAGATAGGGCACGAGGTAC	TGAGTTTTGCTTTGCTGATTCT	55.0	36.4	58.3	57.1	782 bp	1.0	54.2
rps4_p3	GAATGAGATAGGGCACGAGG	TGAGTTTTGCTTTGCTGATTCT	55.0	36.4	57.6	57.1	785 bp	1.0	53.1
rps4_p4	AATGAGATAGGGCACGAGGT	AGTGAATCAAAAAGGAGCCT	50.0	40.0	58.2	54.8	723 bp	1.0	53.1
rps4_p5	AATGAGATAGGGCACGAGGT	TGAAAGTGAATCAAAAAGGAGCC	50.0	39.1	58.2	57.8	727 bp	1.0	52.9