Hotspot

rps11

LSC · 80474-80890 · 417 bp

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Pi 0
MarkerSeek score 25.50
Primer available yes
Label rps11

Feature Metadata

label_name
rps11
feature_type
gene
region
LSC
length_bp
417
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
25.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11_p1 TGGGACCCTTGACACAAATG GGCGGGGTCGAATTATAGTC 50.0 55.0 58.0 57.9 1078 bp 1.0 79.5
rps11_p2 TCGCATTTCGAACAGGCATA GGCGGGGTCGAATTATAGTC 45.0 55.0 58.0 57.9 1285 bp 1.0 79.4
rps11_p3 CACAAATGGATGCGTCCCTA GGCGGGGTCGAATTATAGTC 50.0 55.0 58.0 57.9 1066 bp 1.0 79.2
rps11_p4 TTCAGATGTTGCACGTGTGA GGCGGGGTCGAATTATAGTC 45.0 55.0 58.1 57.9 942 bp 1.0 79.1
rps11_p5 CTTTGGGACCCTTGACACAA GGCGGGGTCGAATTATAGTC 50.0 55.0 57.9 57.9 1081 bp 1.0 79.0