Hotspot

rpl16

LSC · 82764-84419 · 1656 bp

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Pi 0.000403

MarkerSeek score 48.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1656
variable_sites: 1
pi: 0.000403
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCAATGCAAGGGATAATGGA	AGAACAATTCAAATTCTATAAGGT	40.0	25.0	54.4	52.4	1804-1841 bp	1.0	41.7
rpl16_p2	TCAATGCAAGGGATAATGGAA	AGAACAATTCAAATTCTATAAGGT	38.1	25.0	55.2	52.4	1804-1841 bp	1.0	41.7
rpl16_p3	TTCAATGCAAGGGATAATGGA	AGAACAATTCAAATTCTATAAGGT	38.1	25.0	55.2	52.4	1805-1842 bp	1.0	41.7
rpl16_p4	TCAATGCAAGGGATAATGGA	CAAAGAACAATTCAAATTCTATAAGGT	40.0	25.9	54.4	55.2	1807-1844 bp	1.0	41.7
rpl16_p5	TCAATGCAAGGGATAATGGAA	CAAAGAACAATTCAAATTCTATAAGGT	38.1	25.9	55.2	55.2	1807-1844 bp	1.0	41.7