Hotspot

ndhF

SSC · 112477-114729 · 2253 bp

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Pi 0.000888

MarkerSeek score 45.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 3
pi: 0.000888
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CACTTTCAAGAGGCACGCTA	CAGCAGAAGTAGTGAATCGGA	50.0	47.6	58.2	57.5	2371 bp	1.0	66.4
ndhF_p2	CACTTTCAAGAGGCACGCTA	TCAGCAGAAGTAGTGAATCGG	50.0	47.6	58.2	57.5	2372 bp	1.0	66.4
ndhF_p3	CAAGAGGCACGCTATCAAGA	CAGCAGAAGTAGTGAATCGGA	50.0	47.6	57.7	57.5	2365 bp	1.0	65.6
ndhF_p4	CAAGAGGCACGCTATCAAGA	TCAGCAGAAGTAGTGAATCGG	50.0	47.6	57.7	57.5	2366 bp	1.0	65.6
ndhF_p5	TCAAGAGGCACGCTATCAAG	CAGCAGAAGTAGTGAATCGGA	50.0	47.6	57.7	57.5	2366 bp	1.0	65.6