Hotspot

ndhE

SSC · 120231-120536 · 306 bp

Back to Dracula

Pi 0

MarkerSeek score 29.30

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	AGTCTTATGTTTTTCTTTCTATGTGA	CCTTCTATTCTAGTCAACTGAACCA	26.9	40.0	55.2	58.1	426 bp	1.0	40.0
ndhE_p2	AGTCTTATGTTTTTCTTTCTATGTGA	TCCTTCTATTCTAGTCAACTGAACC	26.9	40.0	55.2	57.8	427 bp	1.0	40.0
ndhE_p3	AGTCTTATGTTTTTCTTTCTATGTGA	ACTGAACCAGTTTACTTTTTGTTCA	26.9	32.0	55.2	57.8	410 bp	1.0	40.0
ndhE_p4	GTCTTATGTTTTTCTTTCTATGTGA	CCTTCTATTCTAGTCAACTGAACCA	28.0	40.0	53.9	58.1	425 bp	1.0	40.0
ndhE_p5	AGTCTTATGTTTTTCTTTCTATGTG	CCTTCTATTCTAGTCAACTGAACCA	28.0	40.0	53.9	58.1	426 bp	1.0	40.0