Hotspot

ccsA

SSC · 116528-117514 · 987 bp

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Pi 0.000675
MarkerSeek score 46.70
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
987
variable_sites
1
pi
0.000675
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 AGATTTTAGATTCTATTTAATCCCCC CGGTTCTCAAAAACTTGCACA 30.8 42.9 54.9 57.9 1123 bp 1.0 40.1
ccsA_p2 AGATTTTAGATTCTATTTAATCCCCC ACGGTTCTCAAAAACTTGCAC 30.8 42.9 54.9 57.9 1124 bp 1.0 40.1
ccsA_p3 GATTTTAGATTCTATTTAATCCCCC CGGTTCTCAAAAACTTGCACA 32.0 42.9 53.5 57.9 1122 bp 1.0 40.1
ccsA_p4 GATTTTAGATTCTATTTAATCCCCC ACGGTTCTCAAAAACTTGCAC 32.0 42.9 53.5 57.9 1123 bp 1.0 40.1
ccsA_p5 AGATTTTAGATTCTATTTAATCCCCC ACGGTTCTCAAAAACTTGCA 30.8 40.0 54.9 56.4 1124 bp 1.0 40.1