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clpP1-psbB

LSC · 72609-73369 · 761 bp

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Pi 0.000876

MarkerSeek score 47.40

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: LSC
length_bp: 761
variable_sites: 1
pi: 0.000876
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	935-939 bp	1.0	79.5
clpP1-psbB_p2	TATACGTCAACCCAAGCTGC	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	930-934 bp	1.0	79.4
clpP1-psbB_p3	CTTTTGGAACACCAATGGGC	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.8	57.9	881-885 bp	1.0	78.2
clpP1-psbB_p4	TTTGGAACACCAATGGGCAT	GGCCCAACCAGAAACTAGAG	45.0	55.0	58.3	57.9	879-883 bp	1.0	77.3
clpP1-psbB_p5	TCCAGGACTCCGAAAAGGTA	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.7	57.9	901-905 bp	1.0	77.0