Hotspot

atpB-rbcL

LSC · 53716-54786 · 1071 bp

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Pi 0.0299

MarkerSeek score 65.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1071
variable_sites: 48
pi: 0.0299
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATCTTGCCCGGTGGAAAAAT	ACCAGCTTTAAATCCAACGC	45.0	45.0	58.1	57.0	1238 bp	1.0	74.5
atpB-rbcL_p2	ATCTTGCCCGGTGGAAAAAT	AGCTTTAAATCCAACGCTTGC	45.0	42.9	58.1	58.1	1235 bp	1.0	74.2
atpB-rbcL_p3	ATCTTGCCCGGTGGAAAAAT	GCTTTAAATCCAACGCTTGCT	45.0	42.9	58.1	58.1	1234 bp	1.0	74.2
atpB-rbcL_p4	TGGGCCAATAATTTGAGCGA	ACCAGCTTTAAATCCAACGC	45.0	45.0	57.9	57.0	1206 bp	1.0	74.0
atpB-rbcL_p5	TGGGCCAATAATTTGAGCGA	GCTTTAAATCCAACGCTTGCT	45.0	42.9	57.9	58.1	1202 bp	1.0	73.7