Hotspot

ndhC-trnV-UAC

LSC · 49955-50726 · 772 bp

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Pi 0.00173

MarkerSeek score 47.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 2
pi: 0.00173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCTGGACCTTCCCTAATCGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.2	57.8	921 bp	1.0	76.9
ndhC-trnV-UAC_p2	TCTGGACCTTCCCTAATCGG	AGCCCAATGCAACAAAATGC	55.0	45.0	58.2	58.5	1003 bp	1.0	74.7
ndhC-trnV-UAC_p3	TCTGGACCTTCCCTAATCGG	GAAGGTCTACGGTTCGAGTC	55.0	55.0	58.2	57.5	920 bp	1.0	74.2
ndhC-trnV-UAC_p4	TCTGGACCTTCCCTAATCGG	GTTCGAGTCCGTATAGCCCT	55.0	55.0	58.2	58.7	909 bp	1.0	73.0
ndhC-trnV-UAC_p5	TCTGGACCTTCCCTAATCGG	GAAAGAGCCCAATGCAACAA	55.0	45.0	58.2	57.2	1008 bp	1.0	71.9