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psbK-psbI

LSC · 7058-7562 · 505 bp

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Pi 0.00528

MarkerSeek score 48.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 505
variable_sites: 4
pi: 0.00528
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTGTTTGGCAAGCTGCTT	ACGCCCCGGATCATTAGATA	45.0	50.0	58.1	58.1	627-629 bp	1.0	79.8
psbK-psbI_p2	CTTTGTTTGGCAAGCTGCTT	TACGCCCCGGATCATTAGAT	45.0	50.0	58.1	58.1	628-630 bp	1.0	79.8
psbK-psbI_p3	AATCGTAGACGTTATGCCGG	ACGCCCCGGATCATTAGATA	50.0	50.0	57.9	58.1	675-677 bp	1.0	79.1
psbK-psbI_p4	AATCGTAGACGTTATGCCGG	TACGCCCCGGATCATTAGAT	50.0	50.0	57.9	58.1	676-678 bp	1.0	79.1
psbK-psbI_p5	CGTTATGCCGGTTATCCCTT	ACGCCCCGGATCATTAGATA	50.0	50.0	57.8	58.1	666-668 bp	1.0	78.2