Hotspot

rps11

LSC · 77228-77455 · 228 bp

Back to Dracocephalum

Pi 0.0269

MarkerSeek score 64.00

Primer available yes

Label rps11

Feature Metadata

label_name: rps11
feature_type: gene
region: LSC
length_bp: 228
variable_sites: 22
pi: 0.0269
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	TCGGATTTTACGCGTGTGAT	TCAAGGGGGCTTTATCTGGA	45.0	50.0	58.0	58.0	1229-1271 bp	1.0	91.8
rps11_p2	ATAGACGCGTCACAAGTTCC	TCAAGGGGGCTTTATCTGGA	50.0	50.0	58.0	58.0	1356-1391 bp	0.833333	81.9
rps11_p3	TCGGATTTTACGCGTGTGAT	ATTGACTAAAGGGCTCAGCG	45.0	50.0	58.0	58.0	755-797 bp	0.75	81.6
rps11_p4	TCGGATTTTACGCGTGTGAT	ACCTCAAGGGGGCTTTATCT	45.0	50.0	58.0	58.0	1269 bp	0.083333	43.2
rps11_p5	ATAGACGCGTCACAAGTTCC	ACCTCAAGGGGGCTTTATCT	50.0	50.0	58.0	58.0	1389 bp	0.083333	43.2