Hotspot

accD

LSC · 55898-57364 · 1467 bp

Back to Dracocephalum

Pi 0.0288

MarkerSeek score 74.00

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1467
variable_sites: 161
pi: 0.0288
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AAACCTACGGATCCTTGGGA	AATGAGATTGGGTCCCCTCA	50.0	50.0	58.0	58.0	1907-2590 bp	1.0	91.5
accD_p2	CCCCGGATTTCCACAAAAGA	AATGAGATTGGGTCCCCTCA	50.0	50.0	58.1	58.0	1622-2286 bp	1.0	91.2
accD_p3	TCCCCGGATTTCCACAAAAG	AATGAGATTGGGTCCCCTCA	50.0	50.0	58.1	58.0	1623-2287 bp	1.0	91.2
accD_p4	ACGGATCCTTGGGATTGGTA	AATGAGATTGGGTCCCCTCA	50.0	50.0	58.1	58.0	1901-2584 bp	1.0	90.9
accD_p5	TGTTCGATGCGAAGAGACAG	AATGAGATTGGGTCCCCTCA	50.0	50.0	58.0	58.0	1704-2384 bp	0.916667	88.0