Hotspot

rpl32-trnL-UAG

SSC · 110569-111092 · 524 bp

Back to Dracocephalum

Pi 0.0393

MarkerSeek score 76.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 524
variable_sites: 70
pi: 0.0393
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTTGTTGCTGCCGGAAAAAG	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	1099-1534 bp	0.833333	82.5
rpl32-trnL-UAG_p2	TTTGTTGCTGCCGGAAAAAG	ACTGAAGGGCGGGTACTAAT	45.0	50.0	58.1	58.1	1630-2066 bp	0.833333	82.2
rpl32-trnL-UAG_p3	TTTGTTGCTGCCGGAAAAAG	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.1	57.8	1092-1527 bp	0.833333	82.0
rpl32-trnL-UAG_p4	GGGCAGCGTTAAAAGCATTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	539-878 bp	0.666667	75.4
rpl32-trnL-UAG_p5	GGGCAGCGTTAAAAGCATTT	ACTGAAGGGCGGGTACTAAT	45.0	50.0	57.9	58.1	1074-1406 bp	0.666667	74.8