Hotspot

ndhC-trnV-UAC

LSC · 49012-49961 · 950 bp

Back to Dracocephalum

Pi 0.0272

MarkerSeek score 76.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 950
variable_sites: 99
pi: 0.0272
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGTCATATTCGTAAAGCAAAAACA	AGAAGGTCTACGGTTCGAGT	29.2	50.0	55.9	57.8	806-1016 bp	1.0	52.0
ndhC-trnV-UAC_p2	TGTCATATTCGTAAAGCAAAAACA	GAAGGTCTACGGTTCGAGTC	29.2	55.0	55.9	57.5	805-1015 bp	1.0	52.0
ndhC-trnV-UAC_p3	TGTCATATTCGTAAAGCAAAAACA	GTTCGAGTCCGTATAGCCCT	29.2	55.0	55.9	58.7	794-1004 bp	1.0	52.0
ndhC-trnV-UAC_p4	TGTCATATTCGTAAAGCAAAAACAT	AGAAGGTCTACGGTTCGAGT	28.0	50.0	56.4	57.8	806-1016 bp	1.0	52.0
ndhC-trnV-UAC_p5	GCCAAAATAGGAATAAGACTTGA	AGAAGGTCTACGGTTCGAGT	34.8	50.0	54.7	57.8	852-1062 bp	1.0	51.9