Hotspot

trnT-UGU-trnL-UAA

LSC · 45061-45770 · 710 bp

Back to Dracocephalum

Pi 0.0242
MarkerSeek score 75.60
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
710
variable_sites
68
pi
0.0242
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 752-823 bp 1.0 88.0
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 784-855 bp 1.0 86.9
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 746-817 bp 1.0 86.7
trnT-UGU-trnL-UAA_p4 AAAGCCAGCTATCGGAATCG CCGTAGCGTCTACCGATTTC 50.0 55.0 58.1 58.2 797-868 bp 0.916667 83.8
trnT-UGU-trnL-UAA_p5 AAAGCCAGCTATCGGAATCG CGTCTACCGATTTCGCCATA 50.0 50.0 58.1 57.6 791-862 bp 0.916667 82.5