Hotspot

rps4-trnT-UGU

LSC · 44577-44987 · 411 bp

Back to Dracocephalum

Pi 0.0388

MarkerSeek score 77.80

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 411
variable_sites: 49
pi: 0.0388
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGTTAGTCCCGGTAAAGCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	59.1	57.9	482-540 bp	1.0	82.2
rps4-trnT-UGU_p2	AGTTAGTCCCGGTAAAGCCC	GGTTCGATTCCGATAGCTGG	55.0	55.0	59.1	58.2	441-499 bp	1.0	81.5
rps4-trnT-UGU_p3	AGTTAGTCCCGGTAAAGCCC	CCGCTTAGCTCAGAGGTTAG	55.0	55.0	59.1	57.5	490-548 bp	1.0	79.3
rps4-trnT-UGU_p4	GTTAGTCCCGGTAAAGCCC	CTCAGAGGTTAGAGCATCGC	57.9	55.0	57.5	57.9	481-539 bp	1.0	79.2
rps4-trnT-UGU_p5	AGTTAGTCCCGGTAAAGCCC	ATGGTCATCGGTTCGATTCC	55.0	50.0	59.1	57.8	450-508 bp	0.916667	78.0