Hotspot

ycf3-trnS-GGA

LSC · 43083-43586 · 504 bp

Back to Dracocephalum

Pi 0.0401

MarkerSeek score 78.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 504
variable_sites: 50
pi: 0.0401
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	363-917 bp	1.0	79.8
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	TTAAGTGAGGGTGCGGAAAG	47.6	50.0	58.0	57.8	425-979 bp	1.0	79.6
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	GTGCGGAAAGAGAGGGATTC	47.6	55.0	58.0	58.3	415-969 bp	1.0	78.4
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTG	47.6	50.0	58.0	58.5	365-919 bp	1.0	76.9
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	CAGTTCCAATGCTACGCCTT	47.6	50.0	58.0	58.5	366-920 bp	1.0	76.9