Hotspot

trnE-UUC-trnT-GGU

LSC · 29562-30112 · 551 bp

Back to Dracocephalum

Pi 0.0253
MarkerSeek score 75.00
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
551
variable_sites
59
pi
0.0253
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 649-676 bp 1.0 87.5
trnE-UUC-trnT-GGU_p2 GCTGCCTCCTTGAAAGAGAG CCATGGCGTTACTCTACCAC 55.0 55.0 58.3 58.1 628-655 bp 1.0 87.3
trnE-UUC-trnT-GGU_p3 GCTGCCTCCTTGAAAGAGAG TGGCGTTACTCTACCACTGA 55.0 50.0 58.3 58.1 625-652 bp 1.0 87.2
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG CCGATGACTTACGCCTTACC 55.0 55.0 58.3 58.2 646-673 bp 1.0 86.4
trnE-UUC-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG TTGAACCGATGACTTACGCC 55.0 50.0 58.3 58.3 651-678 bp 1.0 85.2