Hotspot

petN-psbM

LSC · 27581-28527 · 947 bp

Back to Dracocephalum

Pi 0.0331

MarkerSeek score 79.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 947
variable_sites: 111
pi: 0.0331
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGGGAAGAAGTGGGCTTTAG	ACGTAAAAACAGTAAGTCAAAATGA	55.0	28.0	57.9	56.0	957-1012 bp	0.833333	44.3
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	ACGTAAAAACAGTAAGTCAAAATGA	50.0	28.0	58.2	56.0	1020-1075 bp	0.833333	43.8
petN-psbM_p3	ACTCGTAGTGTGGGGAAGAA	ACGTAAAAACAGTAAGTCAAAATGA	50.0	28.0	58.0	56.0	983-1023 bp	0.75	40.6
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	ACGTAAAAACAGTAAGTCAAAATGA	50.0	28.0	58.0	56.0	981-1021 bp	0.75	40.6
petN-psbM_p5	CGTAGTGTGGGGAAGAAGTG	ACGTAAAAACAGTAAGTCAAAATGA	55.0	28.0	57.9	56.0	980-1020 bp	0.75	40.6