Hotspot

trnS-GCU-trnG-UCC

LSC · 7999-8661 · 663 bp

Back to Dracocephalum

Pi 0.0311

MarkerSeek score 77.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 663
variable_sites: 78
pi: 0.0311
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	TACCACTAAACTATACCCGC	50.0	45.0	58.2	54.0	772-819 bp	1.0	58.7
trnS-GCU-trnG-UCC_p2	CAATCCGACGCTTTAGTCCA	TACCACTAAACTATACCCGC	50.0	45.0	58.0	54.0	718-765 bp	0.916667	56.3
trnS-GCU-trnG-UCC_p3	AATCCGACGCTTTAGTCCAC	TACCACTAAACTATACCCGC	50.0	45.0	58.0	54.0	717-764 bp	0.916667	56.3
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	TTACCACTAAACTATACCCGC	50.0	42.9	58.0	54.9	719-766 bp	0.916667	55.2
trnS-GCU-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	TTACCACTAAACTATACCCGC	50.0	42.9	58.0	54.9	718-765 bp	0.916667	55.2