Hotspot

psbI-trnS-GCU

LSC · 7795-7910 · 116 bp

Back to Dracocephalum

Pi 0.0396

MarkerSeek score 80.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 116
variable_sites: 16
pi: 0.0396
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATCTAATGATCCCGGGCGTA	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	218-259 bp	1.0	89.5
psbI-trnS-GCU_p2	TATCTAATGATCCCGGGCGT	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	219-260 bp	1.0	89.5
psbI-trnS-GCU_p3	ATCTAATGATCCCGGGCGTA	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.1	58.0	219-260 bp	1.0	89.5
psbI-trnS-GCU_p4	TATCTAATGATCCCGGGCGT	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.1	58.0	220-261 bp	1.0	89.4
psbI-trnS-GCU_p5	ATCTAATGATCCCGGGCGTA	CAATTGGGTGGGAGAGATGG	50.0	55.0	58.1	57.9	243-284 bp	1.0	88.8