Hotspot

trnH-GUG-psbA

LSC · 130-502 · 373 bp

Back to Dracocephalum

Pi 0.0807

MarkerSeek score 75.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 373
variable_sites: 92
pi: 0.0807
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	AGAAATAGTGGGCGAACGAC	TCAGTGCTATGCATGGTTCC	50.0	50.0	58.0	58.0	853-885 bp	0.25	56.7
trnH-GUG-psbA_p2	AGAAATAGTGGGCGAACGAC	CAGTGCTATGCATGGTTCCT	50.0	50.0	58.0	58.0	852-884 bp	0.25	56.7
trnH-GUG-psbA_p3	AGAAATAGTGGGCGAACGAC	CCCTGTTCAGTGCTATGCAT	50.0	50.0	58.0	58.0	859-891 bp	0.25	56.7
trnH-GUG-psbA_p4	AGAAATAGTGGGCGAACGAC	CTGTTGCGTATTCAGCTCCT	50.0	50.0	58.0	58.0	1054-1086 bp	0.25	56.1
trnH-GUG-psbA_p5	AGAAATAGTGGGCGAACGAC	GGTCGTGAATGGGAGCTTAG	50.0	55.0	58.0	58.1	1103-1135 bp	0.25	55.5