Hotspot

clpP

LSC · 69981-72001 · 2021 bp

Back to Dracaena

Pi 0.0132

MarkerSeek score 54.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2021
variable_sites: 96
pi: 0.0132
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGCACCAACGGAAACCATAA	TACCAATACGCAATGGGAGC	45.0	50.0	58.0	58.0	2702-2736 bp	1.0	83.9
clpP_p2	TTGCACCAACGGAAACCATA	TACCAATACGCAATGGGAGC	45.0	50.0	58.0	58.0	2703-2737 bp	1.0	83.9
clpP_p3	TGCACCAACGGAAACCATAA	TTGTGGGAACGAATGGATGG	45.0	50.0	58.0	58.2	2670-2704 bp	0.636364	67.5
clpP_p4	TGCACCAACGGAAACCATAA	CCCAACCAGAAACTAGAGCC	45.0	55.0	58.0	57.9	2981-2985 bp	0.272727	50.5
clpP_p5	TTGCACCAACGGAAACCATA	CCCAACCAGAAACTAGAGCC	45.0	55.0	58.0	57.9	2982-2986 bp	0.272727	50.5