Hotspot

trnN-GUU-trnR-ACG

IRa · 130079-130674 · 596 bp

Back to Dracaena

Pi 0.00366
MarkerSeek score 64.50
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
596
variable_sites
8
pi
0.00366
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC GAACAGATCCAGTGGAGACG 55.0 55.0 58.0 57.7 771-789 bp 1.0 82.4
trnN-GUU-trnR-ACG_p2 TGGTCGTAGGTTCGAATCCT GAACAGATCCAGTGGAGACG 50.0 55.0 58.2 57.7 722-740 bp 1.0 81.5
trnN-GUU-trnR-ACG_p3 CGGTCGGCTGTTAACTGATT GAACAGATCCAGTGGAGACG 50.0 55.0 58.3 57.7 741-759 bp 1.0 80.4
trnN-GUU-trnR-ACG_p4 TCTCCCGTTGTTCCTCAGTA GAACAGATCCAGTGGAGACG 50.0 55.0 57.7 57.7 775-793 bp 1.0 80.0
trnN-GUU-trnR-ACG_p5 TTCCTCAGTAGCTCAGTGGT GAACAGATCCAGTGGAGACG 50.0 55.0 57.7 57.7 765-783 bp 1.0 80.0