Hotspot

trnR-ACG-trnN-GUU

IRb · 108466-109061 · 596 bp

Back to Dracaena

Pi 0.00366

MarkerSeek score 64.50

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 596
variable_sites: 8
pi: 0.00366
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	771-789 bp	1.0	82.4
trnR-ACG-trnN-GUU_p2	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.7	58.2	722-740 bp	1.0	81.5
trnR-ACG-trnN-GUU_p3	GAACAGATCCAGTGGAGACG	CGGTCGGCTGTTAACTGATT	55.0	50.0	57.7	58.3	741-759 bp	1.0	80.4
trnR-ACG-trnN-GUU_p4	GAACAGATCCAGTGGAGACG	TCTCCCGTTGTTCCTCAGTA	55.0	50.0	57.7	57.7	775-793 bp	1.0	80.0
trnR-ACG-trnN-GUU_p5	GAACAGATCCAGTGGAGACG	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	57.7	57.7	765-783 bp	1.0	80.0