Hotspot

trnW-CCA-trnP-UGG

LSC · 66535-66717 · 183 bp

Back to Dracaena

Pi 0.0147

MarkerSeek score 45.70

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 183
variable_sites: 13
pi: 0.0147
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	CACGGGTTCAAATCCTGTCA	50.0	50.0	57.9	58.1	236-237 bp	1.0	83.4
trnW-CCA-trnP-UGG_p2	GCGTTCTACCGAACTGAACT	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.9	58.1	238-239 bp	1.0	83.3
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	TGTCACGGGTTCAAATCCTG	50.0	50.0	57.9	58.1	239-240 bp	1.0	83.3
trnW-CCA-trnP-UGG_p4	GCGTTCTACCGAACTGAACT	GGGTACAAAATGTCACGGGT	50.0	50.0	57.9	58.1	249-250 bp	1.0	83.2
trnW-CCA-trnP-UGG_p5	GCGTTCTACCGAACTGAACT	TGGGTACAAAATGTCACGGG	50.0	50.0	57.9	58.1	250-251 bp	1.0	83.2