Hotspot

trnF-GAA-ndhJ

LSC · 47897-48620 · 724 bp

Back to Dracaena

Pi 0.00804

MarkerSeek score 63.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 724
variable_sites: 20
pi: 0.00804
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ATGCCAGAAAGTTGGATGGG	50.0	50.0	58.1	58.1	675-839 bp	1.0	84.4
trnF-GAA-ndhJ_p2	CTAGTATGATGCGCGGGAAA	ATGCCAGAAAGTTGGATGGG	50.0	50.0	57.9	58.1	736-900 bp	1.0	83.5
trnF-GAA-ndhJ_p3	GGAAATGGTCGGGATAGCTC	ATGCCAGAAAGTTGGATGGG	55.0	50.0	57.8	58.1	721-885 bp	1.0	83.3
trnF-GAA-ndhJ_p4	AATGGTCGGGATAGCTCAGT	ATGCCAGAAAGTTGGATGGG	50.0	50.0	58.2	58.1	718-882 bp	1.0	83.1
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	ATGCCAGAAAGTTGGATGGG	50.0	50.0	58.2	58.1	717-881 bp	1.0	83.1