Hotspot

petN-psbM

LSC · 28888-29784 · 897 bp

Back to Dracaena

Pi 0.00804

MarkerSeek score 66.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 897
variable_sites: 32
pi: 0.00804
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.0	57.6	996-1075 bp	1.0	75.2
petN-psbM_p2	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	945-1024 bp	1.0	74.4
petN-psbM_p3	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	944-1023 bp	1.0	74.4
petN-psbM_p4	TATGGGGAAGGAGTGGACTC	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.8	57.6	943-1022 bp	1.0	73.9
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	1002-1081 bp	1.0	73.7