Hotspot

ndhF-rpl32

SSC · 110953-111789 · 837 bp

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Pi 0.0323
MarkerSeek score 75.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
837
variable_sites
62
pi
0.0323
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.92
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAATGAAAGGGA ACCCCTTCCTTTTCCAAATGT 47.6 42.9 57.6 57.7 894-962 bp 1.0 67.1
ndhF-rpl32_p2 TGGAAGTGGAATGAAAGGGA ACCCCTTCCTTTTCCAAATGT 45.0 42.9 56.3 57.7 893-961 bp 1.0 65.3
ndhF-rpl32_p3 CTGGAAGTGGAATGAAAGGG ACCCCTTCCTTTTCCAAATGT 50.0 42.9 56.0 57.7 894-962 bp 1.0 62.7
ndhF-rpl32_p4 CTGGAAGTGGAATGAAAGGGAT ACCCCTTCCTTTTCCAAATGT 45.5 42.9 58.0 57.7 894-962 bp 1.0 62.4
ndhF-rpl32_p5 GGAAGTGGAATGAAAGGGATGA ACCCCTTCCTTTTCCAAATGT 45.5 42.9 58.0 57.7 892-960 bp 1.0 62.4