Hotspot

trnF-UUC-ndhJ

LSC · 46585-47205 · 621 bp

Back to Draba

Pi 0.0225
MarkerSeek score 70.50
Primer available yes
Label trnF-UUC-ndhJ

Feature Metadata

label_name
trnF-UUC-ndhJ
feature_type
igs
region
LSC
length_bp
621
variable_sites
40
pi
0.0225
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-UUC-ndhJ_p1 CCTCGTGTCACCAGTTCAAA CGTAAGGATTATATTGCCCCCA 50.0 45.5 58.1 57.7 706-848 bp 1.0 69.2
trnF-UUC-ndhJ_p2 CCTCGTGTCACCAGTTCAAA ACGTAAGGATTATATTGCCCCC 50.0 45.5 58.1 57.7 707-849 bp 1.0 69.2
trnF-UUC-ndhJ_p3 AATCCTCGTGTCACCAGTTC CGTAAGGATTATATTGCCCCCA 50.0 45.5 57.5 57.7 709-851 bp 1.0 66.0
trnF-UUC-ndhJ_p4 AATCCTCGTGTCACCAGTTC ACGTAAGGATTATATTGCCCCC 50.0 45.5 57.5 57.7 710-852 bp 1.0 65.9
trnF-UUC-ndhJ_p5 CCGGGATAGCTCAGTTGGTA CGTAAGGATTATATTGCCCCCA 55.0 45.5 58.6 57.7 744-886 bp 1.0 64.7