Hotspot

psaA-ycf3

LSC · 40570-41307 · 738 bp

Back to Draba

Pi 0.0145
MarkerSeek score 65.30
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
738
variable_sites
32
pi
0.0145
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 TTTTGACTTCTGGTTCCGGC ATTGGTTGACGATCACGAGG 50.0 50.0 58.7 58.0 768-804 bp 1.0 81.7
psaA-ycf3_p2 TTTTGACTTCTGGTTCCGGC AACGCTTACGCCTGGTAATT 50.0 45.0 58.7 57.9 803-839 bp 1.0 80.8
psaA-ycf3_p3 TTTTGACTTCTGGTTCCGGC ACAGGCTATAACGCTTACGC 50.0 50.0 58.7 58.2 812-848 bp 1.0 80.4
psaA-ycf3_p4 TTTTGACTTCTGGTTCCGGC AGCACAGAATTGGTTGACGA 50.0 45.0 58.7 57.7 776-812 bp 1.0 79.7
psaA-ycf3_p5 TTTTGACTTCTGGTTCCGGC AGCCGCTGAGTATTGGAAAC 50.0 50.0 58.7 58.3 830-866 bp 1.0 79.4