Hotspot

trnC-UGC-petN

LSC · 26513-27142 · 630 bp

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Pi 0.0168
MarkerSeek score 67.60
Primer available yes
Label trnC-UGC-petN

Feature Metadata

label_name
trnC-UGC-petN
feature_type
igs
region
LSC
length_bp
630
variable_sites
30
pi
0.0168
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-UGC-petN_p1 TTTCCCCAGTTCAAATCCGG ACTTCTTCCCCACACTACGA 50.0 50.0 58.1 58.0 729-756 bp 1.0 86.0
trnC-UGC-petN_p2 TTTCCCCAGTTCAAATCCGG CACTTCTTCCCCACACTACG 50.0 55.0 58.1 57.9 730-757 bp 1.0 85.4
trnC-UGC-petN_p3 TTTCCCCAGTTCAAATCCGG CTTCCCCACACTACGAGAGA 50.0 55.0 58.1 58.2 725-752 bp 1.0 84.7
trnC-UGC-petN_p4 TTTCCCCAGTTCAAATCCGG TCTTCCCCACACTACGAGAG 50.0 55.0 58.1 58.2 726-753 bp 1.0 84.7
trnC-UGC-petN_p5 TTTCCCCAGTTCAAATCCGG TTCCCCACACTACGAGAGAG 50.0 55.0 58.1 58.2 724-751 bp 1.0 84.7