Hotspot

rpoB-trnC-UGC

LSC · 25500-26441 · 942 bp

Back to Draba

Pi 0.0209
MarkerSeek score 64.70
Primer available yes
Label rpoB-trnC-UGC

Feature Metadata

label_name
rpoB-trnC-UGC
feature_type
igs
region
LSC
length_bp
942
variable_sites
56
pi
0.0209
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-UGC_p1 TCTTTTCCATCCCCGAGCAT CCGGATTTGAACTGGGGAAA 50.0 50.0 59.1 58.1 1024-1052 bp 1.0 76.4
rpoB-trnC-UGC_p2 CTCTTTTCCATCCCCGAGC CCGGATTTGAACTGGGGAAA 57.9 50.0 57.9 58.1 1025-1053 bp 1.0 76.4
rpoB-trnC-UGC_p3 TCTTTTCCATCCCCGAGCAT AAAAGGATTTGCAGTCCCCC 50.0 50.0 59.1 58.4 1006-1034 bp 1.0 74.3
rpoB-trnC-UGC_p4 CTCTTTTCCATCCCCGAGC AAAAGGATTTGCAGTCCCCC 57.9 50.0 57.9 58.4 1007-1035 bp 1.0 74.3
rpoB-trnC-UGC_p5 TTCCCTCTTTTCCATCCCCG CCGGATTTGAACTGGGGAAA 55.0 50.0 59.4 58.1 1029-1057 bp 1.0 74.0