Hotspot

trnR-AGA-atpA

LSC · 8831-9098 · 268 bp

Back to Draba

Pi 0.0366
MarkerSeek score 68.70
Primer available yes
Label trnR-AGA-atpA

Feature Metadata

label_name
trnR-AGA-atpA
feature_type
igs
region
LSC
length_bp
268
variable_sites
27
pi
0.0366
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-AGA-atpA_p1 AGCCTTCCAAGCTAACGATG AGCCCCTCTCACAGTAGAAG 50.0 55.0 58.0 58.1 778-852 bp 1.0 86.4
trnR-AGA-atpA_p2 AGCCTTCCAAGCTAACGATG TGACTGCCTTACCAATCGTG 50.0 50.0 58.0 57.9 1124 bp 0.111111 43.5
trnR-AGA-atpA_p3 TTCTGTGAAAGCACCCAGAC TGACTGCCTTACCAATCGTG 50.0 50.0 58.0 57.9 1320 bp 0.111111 43.4
trnR-AGA-atpA_p4 ACCCTTAGCCTTCCAAGCTA TGACTGCCTTACCAATCGTG 50.0 50.0 58.0 57.9 1130 bp 0.111111 43.4
trnR-AGA-atpA_p5 AAACCCTTAGCCTTCCAAGC TGACTGCCTTACCAATCGTG 50.0 50.0 58.1 57.9 1132 bp 0.111111 43.1