Hotspot

trnS-AGC-trnG-GGA

LSC · 7186-7817 · 632 bp

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Pi 0.0207
MarkerSeek score 66.10
Primer available yes
Label trnS-AGC-trnG-GGA

Feature Metadata

label_name
trnS-AGC-trnG-GGA
feature_type
igs
region
LSC
length_bp
632
variable_sites
37
pi
0.0207
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-AGC-trnG-GGA_p1 CTTTAGTCCGCTCAGCCATC AATCAAACCGAGAGACCCTT 55.0 45.0 58.4 56.5 734-818 bp 1.0 68.9
trnS-AGC-trnG-GGA_p2 CTTTAGTCCGCTCAGCCATC ATCAAACCGAGAGACCCTTT 55.0 45.0 58.4 56.5 733-817 bp 1.0 68.9
trnS-AGC-trnG-GGA_p3 GCAATCCAACGCTTTAGTCC AATCAAACCGAGAGACCCTT 50.0 45.0 57.5 56.5 745-829 bp 1.0 67.8
trnS-AGC-trnG-GGA_p4 GCAATCCAACGCTTTAGTCC ATCAAACCGAGAGACCCTTT 50.0 45.0 57.5 56.5 744-828 bp 1.0 67.8
trnS-AGC-trnG-GGA_p5 CTTTAGTCCGCTCAGCCATC AATCAAACCGAGAGACCCTTT 55.0 42.9 58.4 57.2 734-818 bp 1.0 66.9