Hotspot

trnH-CAC-psbA

LSC · 76-406 · 331 bp

Back to Draba

Pi 0.0533
MarkerSeek score 77.80
Primer available yes
Label trnH-CAC-psbA

Feature Metadata

label_name
trnH-CAC-psbA
feature_type
igs
region
LSC
length_bp
331
variable_sites
52
pi
0.0533
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-CAC-psbA_p1 AATCCACTTGGCTACATCCG GCTGTTGAGGCTCCATCTAC 50.0 55.0 57.7 58.1 382-451 bp 1.0 86.8
trnH-CAC-psbA_p2 AATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.7 57.9 397-466 bp 1.0 86.6
trnH-CAC-psbA_p3 AATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.7 57.9 400-469 bp 1.0 86.6
trnH-CAC-psbA_p4 AATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 50.0 55.0 57.7 58.3 449-518 bp 1.0 84.3
trnH-CAC-psbA_p5 AATCCACTTGGCTACATCCG ACCGTGCTAACCTTGGTATG 50.0 50.0 57.7 57.6 450-519 bp 1.0 83.8