Hotspot

ycf1

SSC · 98146-105069 · 6924 bp

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Pi 0.188

MarkerSeek score 75.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 6924
variable_sites: 1266
pi: 0.188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CAGCGCTTCCTAGTTTAGCA	TTGCTCTGTCCGATTAAGCC	50.0	50.0	58.0	58.0	881 bp	0.5	59.7
ycf1_p2	ACAGCGCTTCCTAGTTTAGC	TTGCTCTGTCCGATTAAGCC	50.0	50.0	58.0	58.0	882 bp	0.5	59.7
ycf1_p3	CAGCGCTTCCTAGTTTAGCA	CCGATTAAGCCTTCTTCCCC	50.0	55.0	58.0	58.0	872 bp	0.5	59.7
ycf1_p4	CAGCGCTTCCTAGTTTAGCA	CGATTAAGCCTTCTTCCCCC	50.0	55.0	58.0	58.0	871 bp	0.5	59.7
ycf1_p5	ACAGCGCTTCCTAGTTTAGC	CGATTAAGCCTTCTTCCCCC	50.0	55.0	58.0	58.0	872 bp	0.5	59.7