Hotspot

rrn23-trnA (UGC)

IRa · 119307-119454 · 148 bp

Back to Downingia

Pi 0.0878

MarkerSeek score 82.60

Primer available yes

Label rrn23-trnA (UGC)

Feature Metadata

label_name: rrn23-trnA (UGC)
feature_type: igs
region: IRa
length_bp: 148
variable_sites: 13
pi: 0.0878
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23-trnA (UGC)_p1	GTCTCTGGGTGCCTAGGTAT	GGTTCGAGTCCGCTTATCTC	55.0	55.0	57.9	57.9	216 bp	1.0	83.1
rrn23-trnA (UGC)_p2	GTCTCTGGGTGCCTAGGTAT	GTTCGAGTCCGCTTATCTCC	55.0	55.0	57.9	57.9	215 bp	1.0	83.1
rrn23-trnA (UGC)_p3	ATTCGGGAATCTCCGGATCT	GGTTCGAGTCCGCTTATCTC	50.0	55.0	58.0	57.9	288 bp	1.0	82.5
rrn23-trnA (UGC)_p4	ATTCGGGAATCTCCGGATCT	GTTCGAGTCCGCTTATCTCC	50.0	55.0	58.0	57.9	287 bp	1.0	82.5
rrn23-trnA (UGC)_p5	ATTCGGGAATCTCCGGATCT	TTCGAGTCCGCTTATCTCCA	50.0	50.0	58.0	58.2	286 bp	1.0	81.6