Hotspot

trnA (UGC)-rrn23

IRb · 93683-93830 · 148 bp

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Pi 0.0878
MarkerSeek score 82.60
Primer available yes
Label trnA (UGC)-rrn23

Feature Metadata

label_name
trnA (UGC)-rrn23
feature_type
igs
region
IRb
length_bp
148
variable_sites
13
pi
0.0878
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnA (UGC)-rrn23_p1 GGTTCGAGTCCGCTTATCTC GTCTCTGGGTGCCTAGGTAT 55.0 55.0 57.9 57.9 216 bp 1.0 83.1
trnA (UGC)-rrn23_p2 GTTCGAGTCCGCTTATCTCC GTCTCTGGGTGCCTAGGTAT 55.0 55.0 57.9 57.9 215 bp 1.0 83.1
trnA (UGC)-rrn23_p3 GGTTCGAGTCCGCTTATCTC ATTCGGGAATCTCCGGATCT 55.0 50.0 57.9 58.0 288 bp 1.0 82.5
trnA (UGC)-rrn23_p4 GTTCGAGTCCGCTTATCTCC ATTCGGGAATCTCCGGATCT 55.0 50.0 57.9 58.0 287 bp 1.0 82.5
trnA (UGC)-rrn23_p5 TTCGAGTCCGCTTATCTCCA ATTCGGGAATCTCCGGATCT 50.0 50.0 58.2 58.0 286 bp 1.0 81.6