Hotspot

pafI-trnS (GGA)

LSC · 47585-48480 · 896 bp

Back to Downingia

Pi 0.122

MarkerSeek score 81.00

Primer available yes

Label pafI-trnS (GGA)

Feature Metadata

label_name: pafI-trnS (GGA)
feature_type: igs
region: LSC
length_bp: 896
variable_sites: 107
pi: 0.122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI-trnS (GGA)_p1	GCCTAACCATTCTTCGTCGT	AGGTACGTTGGGTGAAGGTA	50.0	50.0	58.0	58.0	1473 bp	0.5	59.8
pafI-trnS (GGA)_p2	GCCTAACCATTCTTCGTCGT	TAGGTACGTTGGGTGAAGGT	50.0	50.0	58.0	58.0	1474 bp	0.5	59.8
pafI-trnS (GGA)_p3	TTTTTCCCCTGGAGTTGTCG	AGGTACGTTGGGTGAAGGTA	50.0	50.0	58.0	58.0	1102 bp	0.5	59.7
pafI-trnS (GGA)_p4	TTTTTCCCCTGGAGTTGTCG	TAGGTACGTTGGGTGAAGGT	50.0	50.0	58.0	58.0	1103 bp	0.5	59.7
pafI-trnS (GGA)_p5	GCCTAACCATTCTTCGTCGT	GTTATCGCTGCAAACCCCTA	50.0	50.0	58.0	58.0	1855 bp	0.5	59.7