Hotspot

trnT (GGU)-psbD

LSC · 35160-36240 · 1081 bp

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Pi 0.116
MarkerSeek score 82.70
Primer available yes
Label trnT (GGU)-psbD

Feature Metadata

label_name
trnT (GGU)-psbD
feature_type
igs
region
LSC
length_bp
1081
variable_sites
122
pi
0.116
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT (GGU)-psbD_p1 AGTTCAAAACCGAAAAGGGC TTTACCAAGGGCTATAGTCA 45.0 40.0 57.1 53.0 1123-1155 bp 1.0 49.8
trnT (GGU)-psbD_p2 GTTCAAAACCGAAAAGGGCT TTTACCAAGGGCTATAGTCA 45.0 40.0 57.1 53.0 1122-1154 bp 1.0 49.8
trnT (GGU)-psbD_p3 AGTTCAAAACCGAAAAGGGC TTACCAAGGGCTATAGTCAT 45.0 40.0 57.1 52.8 1122-1154 bp 1.0 49.8
trnT (GGU)-psbD_p4 GTTCAAAACCGAAAAGGGCT TTACCAAGGGCTATAGTCAT 45.0 40.0 57.1 52.8 1121-1153 bp 1.0 49.8
trnT (GGU)-psbD_p5 GTTCAAAACCGAAAAGGGCT TTTACCAAGGGCTATAGTCAT 45.0 38.1 57.1 53.7 1122-1154 bp 1.0 49.8