Hotspot

psbM-trnD (GUC)

LSC · 33590-34510 · 921 bp

Back to Downingia

Pi 0.0929

MarkerSeek score 84.40

Primer available yes

Label psbM-trnD (GUC)

Feature Metadata

label_name: psbM-trnD (GUC)
feature_type: igs
region: LSC
length_bp: 921
variable_sites: 80
pi: 0.0929
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD (GUC)_p1	ACTGCCAGAATATTGACTTCCA	AGATCCCTTAGTTCCCTGGG	40.9	55.0	57.4	57.8	1019-1035 bp	1.0	68.0
psbM-trnD (GUC)_p2	ACTGCCAGAATATTGACTTCCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.4	58.5	992-1008 bp	1.0	66.0
psbM-trnD (GUC)_p3	ACTGCCAGAATATTGACTTCCA	TCCCTTAGTTCCCTGGGATT	40.9	50.0	57.4	57.3	1016-1032 bp	1.0	64.2
psbM-trnD (GUC)_p4	AACTGCCAGAATATTGACTTCCA	AGATCCCTTAGTTCCCTGGG	39.1	55.0	58.1	57.8	1020-1036 bp	1.0	64.0
psbM-trnD (GUC)_p5	ACTGCCAGAATATTGACTTCCA	CCCTTAGTTCCCTGGGATTG	40.9	55.0	57.4	57.3	1015-1031 bp	1.0	63.8