Hotspot

trnS (GCU)-trnG (UCC)

LSC · 13503-14155 · 653 bp

Back to Downingia

Pi 0.113

MarkerSeek score 82.60

Primer available yes

Label trnS (GCU)-trnG (UCC)

Feature Metadata

label_name: trnS (GCU)-trnG (UCC)
feature_type: igs
region: LSC
length_bp: 653
variable_sites: 73
pi: 0.113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS (GCU)-trnG (UCC)_p1	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	776-808 bp	1.0	58.6
trnS (GCU)-trnG (UCC)_p2	ACGAAAACGGAAAGAGAGGG	ACGAATCACACTTTTACCACT	50.0	38.1	57.8	55.5	782-814 bp	1.0	58.4
trnS (GCU)-trnG (UCC)_p3	ACGGAAAGAGAGGGATTCGA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.2	57.3	779-811 bp	1.0	57.5
trnS (GCU)-trnG (UCC)_p4	ACGGAAAGAGAGGGATTCGA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.2	57.3	778-810 bp	1.0	57.5
trnS (GCU)-trnG (UCC)_p5	ACGAAAACGGAAAGAGAGGG	GAACGAATCACACTTTTACCACT	50.0	39.1	57.8	57.3	784-816 bp	1.0	57.3